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Chapters
- 00:04Title
- 01:12Dissection and Preparation of Mouse Seminiferous Tubules
- 03:16Squashing and Labeling Prepared Seminiferous Tubules
- 05:47Analysis and Imaging Tubule Squash Preparations
- 07:03Results: Visualization of Meiotic Progression Using the Tubule Squash Preparation
- 08:35Conclusion
The goal of this tubule squash technique is to rapidly assess cytological features of developing mouse spermatocytes while preserving cellular integrity. This method allows for the study of all stages of spermatogenesis, and can be easily implemented alongside other biochemical and molecular biological approaches for the study of mouse meiosis.
Tags
Keywords: Seminiferous TubuleCytological AnalysisSpermatogenesisMouse ModelSpermatogoniaSpermatocytesSpermatidsMeiosisMale Reproductive BiologySpermatogonial Stem Cell DifferentiationMeiotic Chromosome SegregationPost-meiotic DifferentiationCellular IntegrityInfertilityMammalian SystemsSquash TechniquePBSFixative Lysis SolutionPoly-L-lysine Coated Glass Slide
