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JoVE Journal Developmental Biology
A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
 

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Article DOI: 10.3791/56453-v • 09:40 min • February 6th, 2018
February 6th, 2018 •
*1, *1, 1
1Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health
* These authors contributed equally

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résumé
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The goal of this tubule squash technique is to rapidly assess cytological features of developing mouse spermatocytes while preserving cellular integrity. This method allows for the study of all stages of spermatogenesis, and can be easily implemented alongside other biochemical and molecular biological approaches for the study of mouse meiosis.

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Keywords: Seminiferous Tubule Cytological Analysis Spermatogenesis Mouse Model Spermatogonia Spermatocytes Spermatids Meiosis Male Reproductive Biology Spermatogonial Stem Cell Differentiation Meiotic Chromosome Segregation Post-meiotic Differentiation Cellular Integrity Infertility Mammalian Systems Squash Technique PBS Fixative Lysis Solution Poly-L-lysine Coated Glass Slide
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