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小鼠胚胎心肌内祖群的定量全贴免疫荧光分析
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
DOI:

09:42 min

October 12, 2017

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Chapters

  • 00:05Title
  • 00:55Cardiac Crescent Stage Embryo Harvest and Processing
  • 02:45Immunofluorescence Staining
  • 03:57Embryo Mounting and Confocal Imaging
  • 05:28Image Analysis and Quantitative 3D Modeling
  • 07:49Results: Representative Confocal Image Processing
  • 08:56Conclusion

Summary

Automatic Translation

在这里, 我们描述了一个协议的全贴装免疫荧光和图像定量容积分析早期小鼠胚胎。我们提出这项技术是一个强有力的方法, 定性和定量评估心脏结构在发展过程中, 并提出它可能会广泛适用于其他器官系统。

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