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Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
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Chapters
- 00:05Title
- 00:41Embryo Collection
- 03:25Confocal Imaging
- 04:47Image Analysis and Data Pre-processing
- 08:44Results: Lineage Specification Analysis in Mouse Preimplantation Embryos
- 10:30Conclusion
This protocol presents a method to perform quantitative, single-cell in situ analysis of protein expression to study lineage specification in mouse preimplantation embryos. The procedures necessary for collection of blastocysts, whole-mount immunofluorescent detection of proteins, imaging of samples on a confocal microscope, and nuclear segmentation and image analysis are described.
Tags
Quantitative AnalysisProtein ExpressionLineage SpecificationMouse Preimplantation EmbryosHigh-throughput AnalysisConfocal MicroscopySingle-cell ResolutionSemi-automatedProtein ConcentrationsHeterogeneitiesOpen FlameGlass Pasteur PipetteCapillary EndBlunt EndPregnant Female MouseEmbryonic Day Of DevelopmentAbdominal VisceraUterusCervical EndUterine HornsOviductsOvariesPetri DishPBSDissection MicroscopeOne ML SyringeHypodermic NeedleBlastocysts
