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ミトコンドリアの Ca^{2 +}保持容量測定と Ca^{2 +}-ミトコンドリアの膨潤アッセイをトリガー

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Mitochondrial Ca²⁺ Retention Capacity Assay and Ca²⁺-triggered Mitochondrial Swelling Assay

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ミトコンドリアの Ca^{2 +}保持容量測定と Ca^{2 +}-ミトコンドリアの膨潤アッセイをトリガー

10,751 Views

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05:53 min

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May 01, 2018

DOI:

10.3791/56236-v

DOI:

10.3791/56236-v

•

05:53 min

May 01, 2018

•

16 Views

Wei Li^1,2, Chen Zhang¹, Xiulian Sun³

¹Department of Neurology,Qilu Hospital of Shandong University, ²Department of Neurology,Qingdao Municipal Hospital, ³Brain Research Institute,Qilu Hospital of Shandong University

Summary

このプロトコルが Ca^{2 +}保持能力と Ca^{2 +}を調べる方法を説明することを目的 - トリガー SH SY5Y 分離ミトコンドリアの膨潤ミトコンドリア細胞ステップバイステップ。

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