/

/

Mitochondrial Ca²⁺ Retention Capacity Assay and Ca²⁺-triggered Mitochondrial Swelling Assay

JoVE Journal
Neurociência

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Neurociência

Mitochondrial Ca²⁺ Retention Capacity Assay and Ca²⁺-triggered Mitochondrial Swelling Assay

Mitochondrial Ca²⁺ Retention Capacity Assay and Ca²⁺-triggered Mitochondrial Swelling Assay

10,631 Views

•

05:53 min

•

May 01, 2018

DOI:

10.3791/56236-v

DOI:

10.3791/56236-v

•

05:53 min

May 01, 2018

•

14 Views

Wei Li², Chen Zhang, Xiulian Sun

¹Department of Neurology,Qilu Hospital of Shandong University, ²Department of Neurology,Qingdao Municipal Hospital, ³Brain Research Institute,Qilu Hospital of Shandong University

Tags

Mitochondrial Calcium Retention Capacity Calcium-induced Mitochondrial Swelling Mitochondrial Dysfunction Energy Metabolism SH-SY5Y Cells Mitochondrial Isolation Protease Inhibitor Cell Homogenization Differential Centrifugation Mitochondrial Protein Quantification BCA Assay Calcium Binding Dye Potassium Chloride Media

Read Article