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Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay

Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay

DOI:
10.3791/56236-v

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05:53 min

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May 01, 2018

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, ,
1Department of Neurology,Qilu Hospital of Shandong University, 2Department of Neurology,Qingdao Municipal Hospital, 3Brain Research Institute,Qilu Hospital of Shandong University

Chapters

  • 00:04Title
  • 00:28Isolation of Mitochondria
  • 02:41Determination of Mitochondrial Ca2 Retention Capacity and Ca2-induced Mitochondrial Swelling
  • 04:33Results: The Measurement of Ca2 Retention Capacity and Ca2-induced Mitochondrial Swelling
  • 05:30Conclusion

Summary

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This protocol aims to describe a method to examine the Ca2+ retention capacity and Ca2+- triggered mitochondrial swelling of isolated mitochondria of SH-SY5Y cells step-by-step.

Tags

Mitochondrial Calcium Retention CapacityCalcium-induced Mitochondrial SwellingMitochondrial DysfunctionEnergy MetabolismSH-SY5Y CellsMitochondrial IsolationProtease InhibitorCell HomogenizationDifferential CentrifugationMitochondrial Protein QuantificationBCA AssayCalcium Binding DyePotassium Chloride Media

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