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Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay

Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay

10,748 Views

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05:53 min

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May 01, 2018

DOI:

10.3791/56236-v

DOI:
10.3791/56236-v

•

05:53 min
May 01, 2018

•

16 Views
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1Department of Neurology,Qilu Hospital of Shandong University, 2Department of Neurology,Qingdao Municipal Hospital, 3Brain Research Institute,Qilu Hospital of Shandong University

Summary

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This protocol aims to describe a method to examine the Ca2+ retention capacity and Ca2+- triggered mitochondrial swelling of isolated mitochondria of SH-SY5Y cells step-by-step.

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Cite this Article

Li, W., Zhang, C., Sun, X. Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay. J. Vis. Exp. (135), e56236, doi:10.3791/56236 (2018).

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