Mitochondrial Ca<sup>2+</sup> Retention Capacity Assay and Ca<sup>2+</sup>-triggered Mitochondrial Swelling Assay

Wei Li; Chen Zhang; Xiulian Sun

doi:10.3791/56236

Journal

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Neurosciences

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Mitochondrial Ca²⁺ Retention Capacity Assay and Ca²⁺-triggered Mitochondrial Swelling Assay

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Neurosciences

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Journal JoVE Neurosciences

Mitochondrial Ca²⁺ Retention Capacity Assay and Ca²⁺-triggered Mitochondrial Swelling Assay

10,748 Views

•

05:53 min

•

May 01, 2018

DOI:

10.3791/56236-v

DOI:

10.3791/56236-v

•

05:53 min

May 01, 2018

•

16 Views

Wei Li^1,2, Chen Zhang¹, Xiulian Sun³

¹Department of Neurology,Qilu Hospital of Shandong University, ²Department of Neurology,Qingdao Municipal Hospital, ³Brain Research Institute,Qilu Hospital of Shandong University

Summary

This protocol aims to describe a method to examine the Ca²⁺ retention capacity and Ca²⁺- triggered mitochondrial swelling of isolated mitochondria of SH-SY5Y cells step-by-step.

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Mitochondrial Calcium Retention Capacity Calcium-induced Mitochondrial Swelling Mitochondrial Dysfunction Energy Metabolism SH-SY5Y Cells Mitochondrial Isolation Protease Inhibitor Cell Homogenization Differential Centrifugation Mitochondrial Protein Quantification BCA Assay Calcium Binding Dye Potassium Chloride Media

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Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay

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Mitochondrial Ca²⁺ Retention Capacity Assay and Ca²⁺-triggered Mitochondrial Swelling Assay