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Mitocondriale di Ca2 + ritenzione capacità Assay e Ca2 +-innescato gonfiamento mitocondriale Assay
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Neuroscience
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JoVE Journal Neuroscience
Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay
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Mitocondriale di Ca2 + ritenzione capacità Assay e Ca2 +-innescato gonfiamento mitocondriale Assay

italiano

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10,751 Views

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05:53 min

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May 01, 2018

DOI:

10.3791/56236-v

DOI:
10.3791/56236-v

•

05:53 min
May 01, 2018

•

16 Views
, ,
1Department of Neurology,Qilu Hospital of Shandong University, 2Department of Neurology,Qingdao Municipal Hospital, 3Brain Research Institute,Qilu Hospital of Shandong University

Summary

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Questo protocollo ha lo scopo di descrivere un metodo per esaminare la capacità di ritenzione di Ca2 + e Ca2 +- innescato mitocondriale gonfiore di mitocondri isolati di SH-SY5Y cellule passo-passo.

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Cite this Article

Li, W., Zhang, C., Sun, X. Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay. J. Vis. Exp. (135), e56236, doi:10.3791/56236 (2018).

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