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中規模の<em>ショウジョウバエ</em>プロテオーム実験のための胚抽出物
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Developmental Biology
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JoVE Journal Developmental Biology
Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments
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中規模の<em>ショウジョウバエ</em>プロテオーム実験のための胚抽出物

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7,757 Views

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09:16 min

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May 30, 2017

DOI:

10.3791/55804-v

DOI:
10.3791/55804-v

•

09:16 min
May 30, 2017

•

26 Views
, , , ,
1Biology Department,University of Massachusetts Boston, 2Department of Bioengineering,University of California, San Diego

Summary

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このプロトコールの目標は、中程度の規模（0.5〜1g）でショウジョウバエ胚を採取し、アフィニティー精製 - 質量分析法（AP-MS）のような下流のプロテオーム用途に使用できるタンパク質抽出物を調製するための、 ）。

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Cite this Article

Yang, L., Paul, S., DuBois-Coyne, S., Kyriakakis, P., Veraksa, A. Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments. J. Vis. Exp. (123), e55804, doi:10.3791/55804 (2017).

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