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Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments
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发育生物学
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JoVE 杂志 发育生物学
Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments

Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments

DOI:
10.3791/55804-v

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09:16 min

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May 30, 2017

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, , , ,
1Biology Department,University of Massachusetts Boston, 2Department of Bioengineering,University of California, San Diego

Chapters

  • 00:05Title
  • 01:06Preparation of 5 Liter and 1 Liter Fly Cages and Collection of Fly Embryos
  • 04:12Embryo Dechorionation
  • 05:15Preparation of Embryo Extract
  • 07:15Results: Preparation of Drosophila Embryo Extracts for Proteomics Experiments
  • 08:35Conclusion

Summary

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The goal of this protocol is to provide a straightforward and inexpensive approach to collecting Drosophila embryos at medium scale (0.5-1 g) and preparing protein extracts that can be used in downstream proteomic applications, such as affinity purification-mass spectrometry (AP-MS).

Tags

DrosophilaEmbryoProteomicsProtein ExtractionAffinity PurificationMass SpectrometryCell Signaling发育生物学Medium-scaleUser-friendlyFly CageApple Juice PlatesYeast Paste

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