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中等规模的制备<em>果蝇</em>蛋白质组学实验胚胎提取物
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JoVE Journal Developmental Biology
Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments
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中等规模的制备<em>果蝇</em>蛋白质组学实验胚胎提取物

DOI:
10.3791/55804-v

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09:16 min

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May 30, 2017

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, , , ,
1Biology Department,University of Massachusetts Boston, 2Department of Bioengineering,University of California, San Diego

Chapters

  • 00:05Title
  • 01:06Preparation of 5 Liter and 1 Liter Fly Cages and Collection of Fly Embryos
  • 04:12Embryo Dechorionation
  • 05:15Preparation of Embryo Extract
  • 07:15Results: Preparation of Drosophila Embryo Extracts for Proteomics Experiments
  • 08:35Conclusion

Summary

Automatic Translation

Automatic Translation

该方案的目标是提供一种直接和廉价的方法来收集中等规模的果蝇胚胎（0.5-1 g），并制备可用于下游蛋白质组学应用的蛋白质提取物，如亲和纯化 - 质谱（AP-MS ）。

Tags

DrosophilaEmbryoProteomicsProtein ExtractionAffinity PurificationMass SpectrometryCell SignalingDevelopmental BiologyMedium-scaleUser-friendlyFly CageApple Juice PlatesYeast Paste

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