Journal
/
/
Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments
Journal JoVE
Biologie du développement
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie du développement
Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments

Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments

7,757 Views

09:16 min

May 30, 2017

DOI:

09:16 min
May 30, 2017

26 Views
, , , ,

Summary

Automatically generated

The goal of this protocol is to provide a straightforward and inexpensive approach to collecting Drosophila embryos at medium scale (0.5-1 g) and preparing protein extracts that can be used in downstream proteomic applications, such as affinity purification-mass spectrometry (AP-MS).

Read Article