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Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments

Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments

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09:16 min

May 30, 2017

DOI:

09:16 min
May 30, 2017

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Summary

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The goal of this protocol is to provide a straightforward and inexpensive approach to collecting Drosophila embryos at medium scale (0.5-1 g) and preparing protein extracts that can be used in downstream proteomic applications, such as affinity purification-mass spectrometry (AP-MS).

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