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Developmental Biology
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长期共焦成像根和下胚轴发育的简单室
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development
Please note that all translations are automatically generated.
Click here for the English version.
长期共焦成像根和下胚轴发育的简单室
DOI:
10.3791/55331-v
•
07:59 min
•
May 17, 2017
•
Charlotte Kirchhelle
,
Ian Moore
1
Department of Plant Sciences
,
University of Oxford
Chapters
00:05
Title
00:50
Preparing Seedling Growth Imaging Chambers
04:01
Using the Imaging Chambers
05:40
Results: Root Growth in Chambers Compared to Agar Plates
07:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的是使用高数值孔径目标和全氟萘烷作为浸渍介质,用于高达3天的根和下胚轴发育的高分辨率共焦延时成像的简单技术。
Tags
Confocal Microscopy
Long-term Imaging
Plant Development
Root
Hypocotyl
Organogenesis
Perfluorodecalin
PDMS
Agar
Chamber Design
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