A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

Journal

Biologia do Desenvolvimento

JoVE Journal
Biologia do Desenvolvimento

This content is Free Access.

JoVE Journal Biologia do Desenvolvimento

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

DOI:

10.3791/55331-v

•

07:59 min

•

May 17, 2017

•

Charlotte Kirchhelle, Ian Moore

¹Department of Plant Sciences,University of Oxford

Capítulos

00:05Título
00:50Preparing Seedling Growth Imaging Chambers
04:01Using the Imaging Chambers
05:40Results: Root Growth in Chambers Compared to Agar Plates
07:15Conclusion

Summary

Tadução automática

Presented here is a simple technique for high-resolution confocal time-lapse imaging of root and hypocotyl development for up to 3 days using high numerical-aperture objectives and perfluorodecalin as an immersion medium.

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

•

•

•

Capítulos

Summary

Tadução automática

Tags

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos

Live Confocal Imaging of Developing Arabidopsis Flowers

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

Long-term Live Imaging of Drosophila Eye Disc

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants

Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development

Read Article