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A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

DOI:
10.3791/55331-v

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07:59 min

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May 17, 2017

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,
1Department of Plant Sciences,University of Oxford

Chapters

  • 00:05Title
  • 00:50Preparing Seedling Growth Imaging Chambers
  • 04:01Using the Imaging Chambers
  • 05:40Results: Root Growth in Chambers Compared to Agar Plates
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a simple technique for high-resolution confocal time-lapse imaging of root and hypocotyl development for up to 3 days using high numerical-aperture objectives and perfluorodecalin as an immersion medium.

Tags

Confocal MicroscopyLong-term ImagingPlant DevelopmentRootHypocotylOrganogenesisPerfluorodecalinPDMSAgarChamber Design

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