/
/
طريقة لقياس التمثيل الغذائي في التصنيف القطعان المجتمعات خلية مجمع عن طريق مستقر النظائر للبحث عن المفقودين
A subscription to JoVE is required to view this content. Sign in or start your free trial.
8,098 Views
•
07:41 min
•
February 04, 2017
DOI:
1Department of Medicine, Unit of Computational Medicine,Karolinska Institutet, 2Center for Molecular Medicine,Karolinska Institutet, 3Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry 2,Karolinska Institutet, 4Department of Medicine,University of California San Diego
Summary
Automatically generated
This article describes a method for studying cellular metabolism in complex communities of multiple cell types, using a combination of stable isotope tracing, cell sorting to isolate specific cell types, and mass spectrometry.
Read Article
Cite this Article
Roci, I., Gallart-Ayala, H., Watrous, J., Jain, M., Wheelock, C. E., Nilsson, R. A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing. J. Vis. Exp. (120), e55011, doi:10.3791/55011 (2017).
Copy