/
/
Strukturelle Informationen vom Einzelmolekül-FRET-Experimente unter Verwendung des Fast Nano-Positionierungssystem
This content is Free Access.
11,808 Views
•
12:30 min
•
February 09, 2017
DOI:
Summary
Automatically generated
We present the setup and experimental procedure to obtain smFRET data from large donor-acceptor networks with a TIRF microscope. The step-by-step analysis of these measurements with the Bayesian inference software Fast-NPS yields high-resolved structural information via the application of adapted dye models.
Explore More Videos
Single-molecule FRETFast Nano-positioning SystemStructural BiologyThree-dimensional Probability DistributionProtein DatabaseFluorescence MeasurementsFlow ChamberSample HolderNeutravidinTotal Internal Reflection Fluorescence MicroscopeStage Piezo-motorTIRF-microscopeImmersion OilImmersion LiquidCamera ParametersFile PathAuto-increment Parameters
Read Article
Cite this Article
Dörfler, T., Eilert, T., Röcker, C., Nagy, J., Michaelis, J. Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System. J. Vis. Exp. (120), e54782, doi:10.3791/54782 (2017).
Copy