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Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System

Journal JoVE
Biochimie

This content is Free Access.

Journal JoVE Biochimie

Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System

Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System

11,715 Views

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12:30 min

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February 09, 2017

DOI:

10.3791/54782-v

DOI:

10.3791/54782-v

•

12:30 min

February 09, 2017

•

4 Views

Thilo Dörfler*¹, Tobias Eilert*¹, Carlheinz Röcker, Julia Nagy, Jens Michaelis

¹Institute of Biophysics,Ulm University

Tags

Single-molecule FRET Fast Nano-positioning System Structural Biology Three-dimensional Probability Distribution Protein Database Fluorescence Measurements Flow Chamber Sample Holder Neutravidin Total Internal Reflection Fluorescence Microscope Stage Piezo-motor TIRF-microscope Immersion Oil Immersion Liquid Camera Parameters File Path Auto-increment Parameters

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