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Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
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February 09, 2017
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We present the setup and experimental procedure to obtain smFRET data from large donor-acceptor networks with a TIRF microscope. The step-by-step analysis of these measurements with the Bayesian inference software Fast-NPS yields high-resolved structural information via the application of adapted dye models.
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Single-molecule FRETFast Nano-positioning SystemStructural BiologyThree-dimensional Probability DistributionProtein DatabaseFluorescence MeasurementsFlow ChamberSample HolderNeutravidinTotal Internal Reflection Fluorescence MicroscopeStage Piezo-motorTIRF-microscopeImmersion OilImmersion LiquidCamera ParametersFile PathAuto-increment Parameters
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Dörfler, T., Eilert, T., Röcker, C., Nagy, J., Michaelis, J. Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System. J. Vis. Exp. (120), e54782, doi:10.3791/54782 (2017).
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