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斑马鱼胚胎的倍性操作与热休克治疗2
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Developmental Biology
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JoVE Journal Developmental Biology
Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment
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斑马鱼胚胎的倍性操作与热休克治疗2

DOI:
10.3791/54492-v

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11:19 min

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December 16, 2016

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, , ,
1Laboratory of Genetics,University of Wisconsin, 2Department of Neurobiology,University of Massachusetts Medical School, 3Interdisciplinary Biomedical Graduate Program,University of Pittsburgh School of Medicine

Chapters

  • 00:05Title
  • 00:47Selecting Females for Egg Collection via Interrupted Mating
  • 01:31Preparation of Sperm Solution
  • 02:50UV Treatment of Sperm Solution
  • 03:37Manual Extrusion of Mature Eggs
  • 04:56In Vitro Fertilization
  • 05:51Heat Shock Treatment
  • 07:13Selection of Embryos with a One-cycle Cytokinesis Stall
  • 08:48Results: Heat Shock 2 Treatment of Zebrafish Embryos
  • 09:57Conclusion

Summary

Automatic Translation

Automatic Translation

对于套数操纵修改协议使用热休克诱导胞质一个周期的摊位在早期胚胎。这个协议是表明在斑马鱼，也可以是适用于其它物种。

Tags

Ploidy ManipulationZebrafish EmbryosHeat ShockDiploidizationGeneticsInbred LinesGenetic ManipulationsMatingEgg ExtrusionSperm SolutionUV TreatmentDissectionTestesHank SolutionPipettingUV Lamp

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