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Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos
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JoVE Journal Developmental Biology
Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos

Imaging Intranuclear Actin Rods in Live Heat Stressed Drosophila Embryos

DOI:
10.3791/61297-v

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07:57 min

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May 15, 2020

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, ,
1Integrative Molecular and Biomedical Sciences,Baylor College of Medicine, 2Department of Cell and Molecular Biology, School of Molecular and Cellular Biology,University of Illinois at Urbana-Champaign, 3Verna and Marrs McLean Department of Biochemistry and Molecular Biology,Baylor College of Medicine

Chapters

  • 00:04Introduction
  • 00:51Embryo Collection and Mounting
  • 03:43Injection and Heat Stress to Promote Rod Formation
  • 04:45Imaging of Actin Rods with Confocal Microscopy
  • 06:32Results: Actin Rods in Heat-stressed Embryos
  • 07:17Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to inject Rhodamine-conjugated globular actin into Drosophila embryos and image intranuclear actin rod assembly following heat stress.

Tags

Keywords: Intranuclear Actin RodsLive Heat Stressed Drosophila EmbryosMicroinjectionActin Stress ResponseActin Rod AssemblyMutant ScreenStress ConditionsEmbryo HandlingEmbryo PositioningEmbryo MountingG-actin RedApple Juice AgarDechorionationEmbryo TransferEmbryo AlignmentEmbryo GlueCoverslip Mounting

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