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La manipulación de ploidía de embriones de pez cebra con el Heat Shock Treatment 2
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Developmental Biology
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JoVE Journal Developmental Biology
Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment
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La manipulación de ploidía de embriones de pez cebra con el Heat Shock Treatment 2

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9,849 Views

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11:19 min

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December 16, 2016

DOI:

10.3791/54492-v

DOI:
10.3791/54492-v

•

11:19 min
December 16, 2016

•

2 Views
, , ,
1Laboratory of Genetics,University of Wisconsin, 2Department of Neurobiology,University of Massachusetts Medical School, 3Interdisciplinary Biomedical Graduate Program,University of Pittsburgh School of Medicine

Summary

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Un protocolo modificado para la manipulación de ploidía utiliza un choque de calor para inducir una parada de un ciclo en la citocinesis en el embrión temprano. Este protocolo se demuestra en el pez cebra, pero puede ser aplicable a otras especies.

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Cite this Article

Baars, D. L., Takle, K. A., Heier, J., Pelegri, F. Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment. J. Vis. Exp. (118), e54492, doi:10.3791/54492 (2016).

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