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Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos
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10:18 min
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June 26, 2017
DOI:
1Department of Medical Biotechnology and Translational Medicine,Università degli Studi di Milano, 2Department of Neuroscience; Department of Cell Biology,Howard Hughes Medical Institute, Yale University School of Medicine, 3Program in Cellular Neuroscience, Neurodegeneration and Repair,Yale University School of Medicine, 4Department of BioSciences,Università degli Studi di Milano
Summary
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Protocols described here allow for the study of the electrical properties of excitable cells in the most non-invasive physiological conditions by employing zebrafish embryos in an in vivo system together with a fluorescence resonance energy transfer (FRET)-based genetically encoded voltage indicator (GEVI) selectively expressed in the cell type of interest.
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Benedetti, L., Ghilardi, A., Prosperi, L., Francolini, M., Del Giacco, L. Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos. J. Vis. Exp. (124), e55297, doi:10.3791/55297 (2017).
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