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Biologia do Desenvolvimento
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Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment

Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment

9,849 Views

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11:19 min

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December 16, 2016

DOI:

10.3791/54492-v

DOI:
10.3791/54492-v

•

11:19 min
December 16, 2016

•

2 Views
, , ,
1Laboratory of Genetics,University of Wisconsin, 2Department of Neurobiology,University of Massachusetts Medical School, 3Interdisciplinary Biomedical Graduate Program,University of Pittsburgh School of Medicine

Summary

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A modified protocol for ploidy manipulation uses a heat shock to induce a one-cycle stall in cytokinesis in the early embryo. This protocol is demonstrated in the zebrafish but may be applicable to other species.

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Baars, D. L., Takle, K. A., Heier, J., Pelegri, F. Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment. J. Vis. Exp. (118), e54492, doi:10.3791/54492 (2016).

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