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饲养果蝇<em>果蝇</em>在无菌和悉生条件
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Developmental Biology
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JoVE Journal Developmental Biology
Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions
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饲养果蝇<em>果蝇</em>在无菌和悉生条件

DOI:
10.3791/54219-v

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09:34 min

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July 30, 2016

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, , , , , ,
1Department of Plant and Wildlife Sciences,Brigham Young University, 2Department of Entomology,Cornell University, 3Department of Molecular Biology and Genetics,Cornell University, 4Division of Infectious Diseases, Boston Children’s Hospital,Harvard Medical School, 5Biological Sciences,SUNY Oswego

Chapters

  • 00:05Title
  • 00:43Bacterial Culture and Diet Preparation
  • 02:16Egg-laying Cages
  • 03:13Egg Collection
  • 03:55Egg Dechorionation and Sterile Diet Transfer
  • 05:33Gnotobiotic Fly Generation
  • 06:40Measuring CFU Load and Testing for Sterility
  • 08:05Results: Successful Axenic Fly Rearing Confirmed with CFUs
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

提出了一种用于无菌和限菌条件下饲养果蝇的方法。飞胚dechorionated在次氯酸钠，无菌转移到无菌的饮食，并在密闭容器中饲养。接种饮食和胚胎与细菌导致限菌协会，和细菌的存在是通过电镀全身果蝇匀浆证实。

Tags

Drosophila MelanogasterAxenicGnotobioticMicrobiotaSterile DietMRS AgarLactobacillusAcetobacterGrape Juice AgarYeast PasteEgg Collection

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