Journal
/
발생학
/
Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

20,534 Views

•

09:34 min

•

July 30, 2016

DOI:

10.3791/54219-v

DOI:
10.3791/54219-v

•

09:34 min
July 30, 2016

•

8 Views
, , , , , ,
1Department of Plant and Wildlife Sciences,Brigham Young University, 2Department of Entomology,Cornell University, 3Department of Molecular Biology and Genetics,Cornell University, 4Division of Infectious Diseases, Boston Children’s Hospital,Harvard Medical School, 5Biological Sciences,SUNY Oswego

Summary

Automatically generated

A method for rearing Drosophila melanogaster under axenic and gnotobiotic conditions is presented. Fly embryos are dechorionated in sodium hypochlorite, transferred aseptically to sterile diet, and reared in closed containers. Inoculating diet and embryos with bacteria leads to gnotobiotic associations, and bacterial presence is confirmed by plating whole-body Drosophila homogenates.

Explore More Videos

Drosophila MelanogasterAxenicGnotobioticMicrobiotaSterile DietMRS AgarLactobacillusAcetobacterGrape Juice AgarYeast PasteEgg Collection

Read Article

Cite this Article

Koyle, M. L., Veloz, M., Judd, A. M., Wong, A. C., Newell, P. D., Douglas, A. E., Chaston, J. M. Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions. J. Vis. Exp. (113), e54219, doi:10.3791/54219 (2016).

Download .ris file

Copy

Share Video

.

Copy