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Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster
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Developmental Biology
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JoVE Journal Developmental Biology
Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster

Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster

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10,245 Views

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12:10 min

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April 14, 2017

DOI:

10.3791/55519-v

DOI:
10.3791/55519-v

•

12:10 min
April 14, 2017

•

1 Views
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1Graduate School of Life and Environmental Sciences,University of Tsukuba, 2Life Science Center of Tsukuba Advanced Research Alliance,University of Tsukuba, 3Faculty of Life and Environmental Sciences,University of Tsukuba

Summary

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We describe a protocol for dissection, fixation, and immunostaining of steroidogenic organs in Drosophila larvae and adult females to study steroid hormone biosynthesis and its regulatory mechanism. In addition to steroidogenic organs, we visualize the innervation of steroidogenic organs as well as steroidogenic target cells such as germline stem cells.

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Imura, E., Yoshinari, Y., Shimada-Niwa, Y., Niwa, R. Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster. J. Vis. Exp. (122), e55519, doi:10.3791/55519 (2017).

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