Rearing the Fruit Fly <em>Drosophila melanogaster</em> Under Axenic and Gnotobiotic Conditions

Melinda L. Koyle; Madeline Veloz; Alec M. Judd; Adam C.-N. Wong; Peter D. Newell; Angela E. Douglas; John M. Chaston

doi:10.3791/54219

Journal

/

Biologia do Desenvolvimento

/

Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

JoVE Journal
Biologia do Desenvolvimento

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Biologia do Desenvolvimento

Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

DOI:

10.3791/54219-v

•

09:34 min

•

July 30, 2016

•

Melinda L. Koyle, Madeline Veloz, Alec M. Judd, Adam C.-N. Wong⁴, Peter D. Newell⁵, Angela E. Douglas³, John M. Chaston²

¹Department of Plant and Wildlife Sciences,Brigham Young University, ²Department of Entomology,Cornell University, ³Department of Molecular Biology and Genetics,Cornell University, ⁴Division of Infectious Diseases, Boston Children’s Hospital,Harvard Medical School, ⁵Biological Sciences,SUNY Oswego

Capítulos

00:05Título
00:43Bacterial Culture and Diet Preparation
02:16Egg-laying Cages
03:13Egg Collection
03:55Egg Dechorionation and Sterile Diet Transfer
05:33Gnotobiotic Fly Generation
06:40Measuring CFU Load and Testing for Sterility
08:05Results: Successful Axenic Fly Rearing Confirmed with CFUs
08:49Conclusion

Summary

Tadução automática

A method for rearing Drosophila melanogaster under axenic and gnotobiotic conditions is presented. Fly embryos are dechorionated in sodium hypochlorite, transferred aseptically to sterile diet, and reared in closed containers. Inoculating diet and embryos with bacteria leads to gnotobiotic associations, and bacterial presence is confirmed by plating whole-body Drosophila homogenates.

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Assaying Blood Cell Populations of the Drosophila melanogaster Larva

Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions

Thawing, Culturing, and Cryopreserving Drosophila Cell Lines

Methods to Test Endocrine Disruption in Drosophila melanogaster

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions