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Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions
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Developmental Biology
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JoVE Journal Developmental Biology
Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

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13,934 Views

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10:24 min

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May 24, 2020

DOI:

10.3791/60843-v

DOI:
10.3791/60843-v

•

10:24 min
May 24, 2020

•

32 Views
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1Center for Molecular Medicine,University of Georgia, 2Department of Biochemistry and Molecular Biology, Franklin College of Arts and Sciences,University of Georgia, 3Department of Cellular Biology, Franklin College of Arts and Sciences,University of Georgia

Summary

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In this protocol, we describe a stable, highly efficient differentiation strategy for the generation of postganglionic sympathetic neurons from human pluripotent stem cells. This model will make neurons available for the use of studies of multiple autonomic disorders.

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Cite this Article

Wu, H. F., Zeltner, N. Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions. J. Vis. Exp. (159), e60843, doi:10.3791/60843 (2020).

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