Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow

Yonghong Chen; Azusa Maeda; Jiachuan Bu; Ralph DaCosta

doi:10.3791/54205

Journal

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Immunology and Infection

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Dijbeen Window Chamber Model voor In Vivo Cell Tracking in het Muizen Bone Marrow

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Immunology and Infection

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JoVE Journal Immunology and Infection

Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow

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Dijbeen Window Chamber Model voor In Vivo Cell Tracking in het Muizen Bone Marrow

8,026 Views

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08:41 min

•

July 28, 2016

DOI:

10.3791/54205-v

DOI:

10.3791/54205-v

•

08:41 min

July 28, 2016

•

1 Views

Yonghong Chen*¹, Azusa Maeda*^1,2, Jiachuan Bu¹, Ralph DaCosta^1,2,3

¹Princess Margaret Cancer Centre, ²Department of Medical Biophysics,University of Toronto, ³Techna Institute,University Health Network

Summary

The protocol describes a novel murine femur window chamber model that can be used to track movement of cells in the femoral bone marrow in vivo. Intravital multiphoton fluorescence microscopy is used to image three components of the femoral bone marrow (vasculature, collagen matrix, and neutrophils) over time.

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Dijbeen Window Chamber Model voor<em> In Vivo</em> Cell Tracking in het Muizen Bone Marrow

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Summary

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