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Methyl-binding DNA capture Sequencing for Patient Tissues
JoVE Journal
Genetik
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JoVE Journal Genetik
Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

DOI:
10.3791/54131-v

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08:40 min

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October 31, 2016

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, , , , , , ,
1Department of Molecular Medicine,University of Texas Health Science Center at San Antonio, 2Greehey Children’s Cancer Research Institute,University of Texas Health Science Center at San Antonio

'Bölümler'

  • 00:05'Başlık'
  • 01:18Methyl-binding DNA Capture
  • 02:10Single Fraction Elution
  • 03:08Ethanol Precipitation
  • 04:29Sequencing
  • 05:58Results: Sequencing of Methyl-binding DNA Capture
  • 07:15Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Here we present a protocol to investigate genome wide DNA methylation in large scale clinical patient screening studies using the Methyl-Binding DNA Capture sequencing (MBDCap-seq or MBD-seq) technology and the subsequent bioinformatics analysis pipeline.

'Etiketler'

DNA MethylationEkolojiPatient CohortsBiomarkersMethyl-binding DNA CaptureSequencingGenome-wide AnalysisCost-effectiveSonicationFragment SizeElutionMagnetic Beads

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