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Isolamento de leucócitos de infiltração de rato pele utilizando digestão enzimática e separação por gradiente
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

Isolamento de leucócitos de infiltração de rato pele utilizando digestão enzimática e separação por gradiente

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20,897 Views

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07:11 min

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January 25, 2016

DOI:

10.3791/53638-v

DOI:
10.3791/53638-v

•

07:11 min
January 25, 2016

•

10 Views
, , ,
1Department of Biology,Macalester College, 2Department of Medicine, Division of Rheumatic and Autoimmune Diseases, Center for Immunology,University of Minnesota

Summary

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This protocol describes enzymatic digestion of mouse skin in nutrient-rich medium followed by gradient separation to isolate leukocytes. Cells thus derived can be used for diverse downstream applications. This is an effective, economical, and improved alternative to tissue dissociation machines and harsher trypsin and dispase-based tissue digestion protocols.

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Cite this Article

Benck, C. J., Martinov, T., Fife, B. T., Chatterjea, D. Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation. J. Vis. Exp. (107), e53638, doi:10.3791/53638 (2016).

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