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Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation
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JoVE Journal 免疫学と感染
Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

20,897 Views

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07:11 min

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January 25, 2016

DOI:

10.3791/53638-v

DOI:
10.3791/53638-v

•

07:11 min
January 25, 2016

•

10 Views
, , ,
1生物学科,Macalester College, 2Department of Medicine, Division of Rheumatic and Autoimmune Diseases, Center for Immunology,University of Minnesota

概要

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This protocol describes enzymatic digestion of mouse skin in nutrient-rich medium followed by gradient separation to isolate leukocytes. Cells thus derived can be used for diverse downstream applications. This is an effective, economical, and improved alternative to tissue dissociation machines and harsher trypsin and dispase-based tissue digestion protocols.

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Cite this Article

Benck, C. J., Martinov, T., Fife, B. T., Chatterjea, D. Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation. J. Vis. Exp. (107), e53638, doi:10.3791/53638 (2016).

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