Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Isolation of Infiltrating Leukocytes from Mouse…
JoVE Journal
Immunology and Infection

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Journal Immunology and Infection
Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation
 

Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

Article DOI: 10.3791/53638-v • 07:11 min • January 25th, 2016
January 25th, 2016 •
1, 2, 2, 1
1Department of Biology, Macalester College, 2Department of Medicine, Division of Rheumatic and Autoimmune Diseases, Center for Immunology, University of Minnesota

Capítulos

Resumen
Automatic Translation

This protocol describes enzymatic digestion of mouse skin in nutrient-rich medium followed by gradient separation to isolate leukocytes. Cells thus derived can be used for diverse downstream applications. This is an effective, economical, and improved alternative to tissue dissociation machines and harsher trypsin and dispase-based tissue digestion protocols.

Tags

Isolation Infiltrating Leukocytes Mouse Skin Enzymatic Digest Gradient Separation Cellular Mechanisms Molecular Mechanisms Mouse Models Skin Pathologies Atopic Dermatitis Resident Cell Populations Skin Harvesting Adipose Tissue Connective Tissue Visual Demonstration Precision Practice Surgical Scissors Flank Skin ND4 Swiss-Webster Mouse Surgical Blade Scraping Away Fat And Connective Tissue 50 Milliliter Conical Tube HBSS EDTA HEPES FBS Incubation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter