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Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

20,788 Views

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07:11 min

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January 25, 2016

DOI:

10.3791/53638-v

DOI:
10.3791/53638-v

•

07:11 min
January 25, 2016

•

3 Views
, , ,
1Department of Biology,Macalester College, 2Department of Medicine, Division of Rheumatic and Autoimmune Diseases, Center for Immunology,University of Minnesota

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LeukocytesInfiltrationSkinEnzymatic DigestGradient SeparationMouse ModelAtopic DermatitisSkin PathologiesHBSSEDTAHEPESFBSCollagenase DDensity Gradient

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Benck, C. J., Martinov, T., Fife, B. T., Chatterjea, D. Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation. J. Vis. Exp. (107), e53638, doi:10.3791/53638 (2016).

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