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Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation
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Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

20,788 Views

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07:11 min

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January 25, 2016

DOI:

10.3791/53638-v

DOI:
10.3791/53638-v

•

07:11 min
January 25, 2016

•

3 Views
, , ,
1Department of Biology,Macalester College, 2Department of Medicine, Division of Rheumatic and Autoimmune Diseases, Center for Immunology,University of Minnesota

Tags

LeukocytesInfiltrationSkinEnzymatic DigestGradient SeparationMouse ModelAtopic DermatitisSkin PathologiesHBSSEDTAHEPESFBSCollagenase DDensity Gradient

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Benck, C. J., Martinov, T., Fife, B. T., Chatterjea, D. Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation. J. Vis. Exp. (107), e53638, doi:10.3791/53638 (2016).

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