Journal
/
Immunology and Infection
/
Monitoring Extracellular pH in Cross-Kingdom Biofilms using Confocal Microscopy
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Monitoring Extracellular pH in Cross-Kingdom Biofilms using Confocal Microscopy

Monitoring Extracellular pH in Cross-Kingdom Biofilms using Confocal Microscopy

DOI:
10.3791/60270-v

•

10:26 min

•

January 30, 2020

•

,
1Department of Dentistry and Oral Health,Aarhus University

Chapters

  • 00:05Title
  • 01:17Cultivation of Cross-kingdom Biofilms
  • 03:35Ratiometric pH Imaging
  • 05:07Calibration of the Ratiometric Dye
  • 06:15Digital Image Analysis
  • 08:38Results: pH Ratiometry in Cross-kingdom Biofilms Exposed to Glucose
  • 09:40Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol describes the cultivation of cross-kingdom biofilms consisting of Candida albicans and Streptococcus mutans and presents a confocal microscopy-based method for the monitoring of extracellular pH inside these biofilms.

Tags

Cross-kingdom BiofilmsStreptococcus MutansCandida AlbicansConfocal MicroscopyPH RatiometryExtracellular MatrixMicrobial MetabolismBiofilm GrowthSalivary SolutionFetal Bovine SerumBrain Heart InfusionSucrose

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Neutrophil Extracellular Traps: How to Generate and Visualize Them

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Ratiometric Imaging of Extracellular pH in Dental Biofilms

Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

Read Article