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A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics

A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics

English

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1,801 Views

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08:30 min

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September 16, 2022

DOI:

10.3791/64041-v

DOI:
10.3791/64041-v

•

08:30 min
September 16, 2022

•

7 Views
, ,
1Sheffield Collaboratorium for Antimicrobial Resistance and Biofilms (SCARAB),University of Sheffield, 2Department of Chemical and Biological Engineering,University of Sheffield

Summary

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The protocol describes a step-by-step method to set up an ex vivo ovine wounded skin model infected with Staphylococcus aureus. This high-throughput model better simulates infections in vivo compared with conventional microbiology techniques and presents researchers with a physiologically relevant platform to test the efficacy of emerging antimicrobials.

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Cite this Article

Regan, H. C., Taylor, A. F., Karunakaran, E. A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics. J. Vis. Exp. (187), e64041, doi:10.3791/64041 (2022).

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