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Laser-guided Neuronal Tracing In Brain Explants
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November 25, 2015
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We describe a technique to label neurons and their processes via anterograde or retrograde tracer injections into brain nuclei using an in vitro preparation. We modified an existing method of in vitro tracer electroporation by taking advantage of fluorescently labeled mouse mutants and basic optical equipment in order to increase labeling accuracy.
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Albrecht, O., Klug, A. Laser-guided Neuronal Tracing In Brain Explants. J. Vis. Exp. (105), e53333, doi:10.3791/53333 (2015).
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