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JoVE Journal Neuroscience
Laser-guided Neuronal Tracing In Brain Explants
 

Laser-guided Neuronal Tracing In Brain Explants

Article DOI: 10.3791/53333-v • 06:40 min • November 25th, 2015
November 25th, 2015 •
1, 1
1Department of Physiology and Biophysics, University of Colorado School of Medicine

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We describe a technique to label neurons and their processes via anterograde or retrograde tracer injections into brain nuclei using an in vitro preparation. We modified an existing method of in vitro tracer electroporation by taking advantage of fluorescently labeled mouse mutants and basic optical equipment in order to increase labeling accuracy.

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Laser-guided Neuronal Tracing Brain Explants In Vitro Tracer Injection Electroporation Laser Illumination Filter Goggles Fluorescent Genetic Markers Band-passing Filter Goggles Accuracy Of Injections Dye-spread/uptake Laser Illumination Technique Neurocircuit Functionality GFP Expression
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