È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Laser-guided Neuronal Tracing In Brain Explants
Capitoli
- 00:05Titolo
- 00:56Optical Genotyping
- 02:25Preparing for the Tracer Injections
- 03:05Anterograde/Retrograde Tracing
- 05:01Results: Labeling Connections within the Trapezoid Body
- 05:49Conclusion
We describe a technique to label neurons and their processes via anterograde or retrograde tracer injections into brain nuclei using an in vitro preparation. We modified an existing method of in vitro tracer electroporation by taking advantage of fluorescently labeled mouse mutants and basic optical equipment in order to increase labeling accuracy.
