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Laser-guided Neuronal Tracing In Brain Explants
JoVE Journal
神経科学
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JoVE Journal 神経科学
Laser-guided Neuronal Tracing In Brain Explants

Laser-guided Neuronal Tracing In Brain Explants

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06:40 min

November 25, 2015

DOI:

06:40 min
November 25, 2015

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概要

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We describe a technique to label neurons and their processes via anterograde or retrograde tracer injections into brain nuclei using an in vitro preparation. We modified an existing method of in vitro tracer electroporation by taking advantage of fluorescently labeled mouse mutants and basic optical equipment in order to increase labeling accuracy.

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