Journal
/
Environment
/
Metodo EPA 1615. Misura di Enterovirus e Norovirus Presenza in Water di Cultura e RT-qPCR. Parte III. Rilevamento virus mediante RT-qPCR
JoVE Journal
Environment
This content is Free Access.
JoVE Journal Environment
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
Please note that all translations are automatically generated. Click here for the English version.

Metodo EPA 1615. Misura di Enterovirus e Norovirus Presenza in Water di Cultura e RT-qPCR. Parte III. Rilevamento virus mediante RT-qPCR

italiano

Automatically Generated

12,250 Views

•

12:32 min

•

January 16, 2016

DOI:

10.3791/52646-v

DOI:
10.3791/52646-v

•

12:32 min
January 16, 2016

•

12 Views
, , , , ,
1National Exposure Research Laboratory,U.S. Environmental Protection Agency, 2Technical Services Center, Office of Ground Water and Drinking Water,U.S. Environmental Protection Agency

Summary

Automatically generated

Here we present a procedure to quantify enterovirus and norovirus in environmental and drinking waters using reverse transcription-quantitative PCR. Mean virus recovery from groundwater with this standardized procedure from EPA Method 1615 was 20% for poliovirus and 30% for murine norovirus.

Explore More Videos

EPA Method 1615EnterovirusNorovirusRT-qPCRVirus DetectionWater SamplesCentrifugal UltrafiltrationNucleic Acid ExtractionReverse TranscriptionReal-time Thermal CyclerStandard CurveEnvironmental MicrobiologyRisk AssessmentDrinking WaterRecreational Water

Read Article

Cite this Article

Fout, G. S., Cashdollar, J. L., Griffin, S. M., Brinkman, N. E., Varughese, E. A., Parshionikar, S. U. EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR. J. Vis. Exp. (107), e52646, doi:10.3791/52646 (2016).

Download .ris file

Copy

Share Video

.

Copy