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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
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Medio ambiente
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JoVE Revista Medio ambiente
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

12,250 Views

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12:32 min

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January 16, 2016

DOI:

10.3791/52646-v

DOI:
10.3791/52646-v

•

12:32 min
January 16, 2016

•

12 Views
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1National Exposure Research Laboratory,U.S. Environmental Protection Agency, 2Technical Services Center, Office of Ground Water and Drinking Water,U.S. Environmental Protection Agency

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Here we present a procedure to quantify enterovirus and norovirus in environmental and drinking waters using reverse transcription-quantitative PCR. Mean virus recovery from groundwater with this standardized procedure from EPA Method 1615 was 20% for poliovirus and 30% for murine norovirus.

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EPA Method 1615EnterovirusNorovirusRT-qPCRVirus DetectionWater SamplesCentrifugal UltrafiltrationNucleic Acid ExtractionReverse TranscriptionReal-time Thermal CyclerStandard CurveEnvironmental MicrobiologyRisk AssessmentDrinking WaterRecreational Water

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Fout, G. S., Cashdollar, J. L., Griffin, S. M., Brinkman, N. E., Varughese, E. A., Parshionikar, S. U. EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR. J. Vis. Exp. (107), e52646, doi:10.3791/52646 (2016).

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