/
/
Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
A subscription to JoVE is required to view this content. Sign in or start your free trial.
6,249 Views
•
07:25 min
•
June 25, 2020
DOI:
1Department of Plant Pathology, Coastal Plain Experiment Station,University of Georgia, 2University of Georgia Cooperative Extension, 3Department of Plant Pathology, Tropical Research & Education Center,University of Florida, 4Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center,Tennessee State University
Summary
Automatically generated
We developed a method to detect Phytophthora capsici zoospores in water sources using a filter paper DNA extraction method coupled with a loop-mediated isothermal amplification (LAMP) assay that can be analyzed in the field or in the lab.
Read Article
Cite this Article
Hudson, O., Waliullah, S., Hand, J., Gazis-Seregina, R., Baysal-Gurel, F., Ali, M. E. Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification. J. Vis. Exp. (160), e61478, doi:10.3791/61478 (2020).
Copy