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Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice
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JoVE Journal 免疫学と感染
Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

DOI:
10.3791/52607-v

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11:44 min

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March 24, 2015

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, , , , , , , , , , ,
1Department of Medicine III,RWTH University-Hospital Aachen, 2IZKF Aachen Core Facility "Two-Photon Imaging",RWTH University-Hospital Aachen, 3Institute for Laboratory Animal Science & Experimental Surgery,RWTH Aachen University, 4Institute for Pharmacology,RWTH University-Hospital Aachen

章

  • 00:05標題
  • 02:50Tracheotomy
  • 04:36Laparatomy
  • 05:50Sample Setup
  • 08:39Results: Intravital TPLSM with CCI4 Damaged Livers
  • 10:02Conclusion

概要

自動翻訳

自動翻訳

Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.

タグ

Intravital MicroscopyMultiphoton ImagingLiver InflammationImmune CellsCXCR6.Gfp Reporter MiceLeukocyte MigrationLiver InjuryCarbon Tetrachloride

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