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Immunology and Infection
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内皮细胞,细胞连接实时成像过程中中性粒细胞轮回在生理流动
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow
Please note that all translations are automatically generated.
Click here for the English version.
内皮细胞,细胞连接实时成像过程中中性粒细胞轮回在生理流动
DOI:
10.3791/51766-v
•
11:26 min
•
August 14, 2014
•
Jeffrey Kroon*
1
,
Anna E. Daniel*
1
,
Mark Hoogenboezem
,
Jaap D. van Buul
1
Department of Molecular Cell Biology
,
Sanquin Research and Landsteiner Laboratory, AMC at University of Amsterdam
Chapters
00:05
Title
01:59
Plating and Maintenance of Human Umbilical Vein Endothelial Cells
04:00
Polymorphonuclear Leukocyte Isolation Using Percoll Gradients
06:36
Labeling of Endothelial Junctional VE-cadherin and PECAM-1 and Performing the PMN TEM Assay Under Flow
09:24
Results: Visualization of the Dynamics of VE-cadherin and PECAM-1 During Neutrophil TEM
10:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
白细胞交叉使用旁或跨细胞途径的单层内皮细胞。我们开发了一个简单的试验,以遵循内源交界的分布VE-cadherin和PECAM-1白细胞跨内皮迁移过程中的生理流动下两路轮回区分。
Tags
Endothelial Cell-cell Junctions
Neutrophil Transmigration
Physiological Flow
Leukocyte Transendothelial Migration
Paracellular Route
Transcellular Route
VE-cadherin
PECAM-1
In Vitro Assay
Fluorescent Antibodies
Electrical Cell-substrate Impedance Sensing
FRAP Measurements
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