/
/
Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow
This content is Free Access.
Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow
19,247 Views
•
11:26 min
•
August 14, 2014
DOI:
Summary
Automatically generated
Leukocytes cross the endothelial monolayer using the paracellular or the transcellular route. We developed a simple assay to follow the distribution of endogenous junctional VE-cadherin and PECAM-1 during leukocyte transendothelial migration under physiological flow to discriminate between the two transmigration routes.
Read Article
Cite this Article
Kroon, J., Daniel, A. E., Hoogenboezem, M., van Buul, J. D. Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow. J. Vis. Exp. (90), e51766, doi:10.3791/51766 (2014).
Copy