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Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

English

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8,942 Views

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08:09 min

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June 19, 2019

DOI:

10.3791/59617-v

DOI:
10.3791/59617-v

•

08:09 min
June 19, 2019

•

9 Views
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1Division of Inflammation Biology,La Jolla Institute for Immunology, 2Flow Cytometry Core Facility,La Jolla Institute for Immunology

Summary

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Here, we present a protocol to process fresh bone marrow (BM) isolated from mouse or human for high-dimensional mass cytometry (Cytometry by Time-Of-Flight, CyTOF) analysis of neutrophil-lineage cells.

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Cite this Article

Zhu, Y. P., Padgett, L., Dinh, H. Q., Marcovecchio, P., Wu, R., Hinz, D., Kim, C., Hedrick, C. C. Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells. J. Vis. Exp. (148), e59617, doi:10.3791/59617 (2019).

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